Overexpressed Hsa_circ_0001326 Contributes to the Decreased Cell Viability in SWAN71 Cells by Regulating MiR-186-5p/p27 Kip1 Axis

Preeclampsia (PE) is a severe pregnancy-specific complication responsible for majority of maternal and fetal mortality. The dysfunction trophoblast cells known to be associated with the etiology PE. Moreover, elevated expression hsa_circ_0001326 was found in patients PE without elucidating specific mechanisms. Thus, this study aimed investigate role vitro. Human SWAN71 were used study. Cell proliferation, apoptosis cell cycle detected by 5-ethynyl-2?-deoxyuridine (EdU) staining, counting kit-8 assay, Annexin V/propidium iodide (PI) staining flow cytometry, respectively. Dual luciferase assay performed validate predicted targets. Additionally, Western blot conducted protein detection. results indicated overexpression (OE) remarkably decreased viability proliferation cells. MiR-186-5p identified as target hsa_circ_0001326. Meanwhile, p27 Kip1 validated hsa_miR-186-5p. increased migration induced OE inhibited knockdown. Hsa_circ_0001326 upregulated cleaved caspase3 downregulated CDK2 cyclin E1 cells, while these phenomena reversed In addition, G0/G1 arrest also attenuated presence Taken together, contributed targeting miR-186-5p via upregulation Kip1. Our findings helpful uncover pathophysiological process PE, well inspire development novel targeted therapy against